Applications of Molecular Microbiological Methods. Applications of Molecular Microbiological Methods | Book"A must for scientists in oil field companies" (Fungal Diversity)Publisher: Caister Academic Press.
Edited by: Torben L. Skovhus, Sean M. Caffrey and Casey R. J. Hubert. Det Norske Veritas (DNV), Bergen, Norway; Genome Alberta, Calgary, Canada; Newcastle University, Newcastle, UK; respectively. Pages: xii + 2. 14. Hardback: Publication date: March 2. Buy book. ISBN: 9.
Price: GB £1. 59 or US $3. Ebook: Publication date: January 2. Buy ebook. ISBN: 9. Price: GB £1. 59 or US $3. Customers who viewed this book also viewed: Innovative, constructive and continually evolving technologies are propelling microbiology into an exciting new era. This new era will witness the harnessing and control of complex microbial communities in a huge variety of applications in the industrial, medical and environmental spheres. State- of- the- art tools are being developed and utilized to analyse the molecules that microorganisms possess and generate, including DNA, RNA, proteins, lipids and cellular metabolites.
This eBook will help you understand the ways that chromosomes are linked to some human diseases and. Rotavirus and Intestinal Bacterial Infections. Cholesterol. Blood Pressure. Liver Disease. Gum Disease and Dental Caries (Cavities) Bacterial Infections. Irritable Bowel Syndrome and Crohn’s. Digestion Problems. Allergies. Attention Subscribers After 5/24/2013. The 5th edition of Through the Microscope is now finished and available as a website subscription, as an ebook and as a hard copy from lulu.com. For subscribers to the 4th edition who are.
In addition to systemic and gastrointestinal involvement, Crohn's disease can affect many other organ systems. Inflammation of the interior portion of the eye, known as uveitis, can cause blurred vision and eye pain. ORAL PROBIOTICS: Fighting Tooth Decay, Periodontal Disease and Airway Infections Using Friendly Bacteria. by Case Adams, Naturopath ·. More people are affected by Lyme disease each year than breast cancer, yet we rarely think about Lyme as the cause of our medical maladies. Your host, Dr. Jay Davidson, nearly lost his wife to chronic Lyme disease, which. Conflict of interest statement. The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The oral cavity supports a rich and diverse microbial population. Oral health is dependent on the maintenance of stable microbial communities; disease occurs when this balance is disturbed and more pathogenic species outgrow.
This book, written by international experts in the field, presents emerging molecular methods that allow the diversity of a microbial community to be surveyed and its functions to be investigated. The first part of the book provides examples of the application of molecular microbiological methods in various industrial applications. Part two deals with the identification of microorganisms in medical settings while the third part presents case studies that use molecular methods to assess the structure and function of microbial communities in natural environments. The fourth part of the book describes in greater detail the methods and technologies featured in the preceding case studies including metagenomics, stable isotope probing, fluorescence in situ hybridization, quantitative PCR, reverse transcription PCR and single cell methods.
These detailed descriptions enable readers to evaluate the applicability of various tools for approaching questions and case studies of their own. This practical, authoritative and up- to- date volume is a valuable resource for anyone applying or developing molecular methods in any area of microbiology and is a recommended acquisition for all microbiology laboratories. Reviews"This book enlightens the readers with recent and current molecular techniques with case studies which can be used to resolve the challenges encountered in their field of microbiology .. A valuable guide for molecular microbiologists and molecular studies based laboratories. A must for scientists in oil field companies research and development and for quality control sections in other industries."from. Fungal Diversity (December 2.
Table of contents. Molecular Methods in Microbiologically Influenced Corrosion: Research, Monitoring and Control. Gerard Muyzer and Florence Marty. Microbiologically Influenced Corrosion (MIC) is an enormous problem in different industrial sectors with large economical consequences. Although research has been performed on microorganisms associated with MIC, the causative microbes have not been identified yet.
Ranked #1 in citations in Psychology, Neurosciences, Plant Sciences, Immunology, Neurology and Physiology. Ranked #2 in citations in Pharmacology & Pharmacy and Microbiology. Journals with impact factors on average ranked in.
Traditional microbiological techniques have been used, but they only detect 1% of the microbes in nature and so are not suited for this purpose. Molecular methods, based on DNA and RNA, are more efficient to characterize microbial communities in different environments, as they are sensitive and reliable, and not dependent on cultivation. In this chapter we give an overview of nucleic acid- based molecular methods that have been used to study microbial communities associated with MIC and give an outlook on the use of novel, state- of- the art methods that might be applied in the future. Using the Power of Molecular Microbiological Methods in Oilfield Corrosion Management to Diagnose MICVictor V. Keasler and Indranil Chatterjee.
It is well established that microbes present in oilfield systems can cause several problems for operators such as hydrogen sulfide (H2. S) production, microbiologically influenced corrosion (MIC), and/or biofouling. However, it is still being debated as to exactly what types of organisms actually cause these problems and whether their presence alone is enough to prove that the associated problems are going to be encountered. This chapter highlights two different case histories where microbes were previously believed to be of minimal risk based on culture- based enumeration. However, molecular characterization revealed elevated microbial numbers as well as a potential high risk species in both systems as the predominant sessile organism and a potential significant asset integrity risk.
Interestingly, the outcome with regards to localized corrosion was very different between the two systems. This chapter is a reminder that the data obtained through molecular analysis is most valuable when it is correlated back to a system key performance indicator (KPI) to enable smart decisions. Next Generation Sequencing Approach to Understand Pipeline Biocorrosion. Hyung Soo Park, Jaspreet Mand, Thomas R. Jack and Gerrit Voordouw. Use of pyrotag sequencing to determine microbial community compositions associated with corrosion in a pipeline system handling brackish water has shown that different populations are present in different parts of a single pipeline and treatment system. Possible factors influencing the composition of the microbial population include the source of water, flow, time of sampling, mode of growth (planktonic or sessile) and addition of treatment chemicals.
Detailed examination of the populations indicated that methanogenic organisms may be responsible for MIC in the upstream water gathering system but downstream of the injection point for sodium bisulfite (an oxygen scavenger) Deltaproteobacteria are more likely the cause of MIC. The mechanism of attack is not necessarily restricted to the widely recognized action of SRB but may also be due to elemental sulfur formed by Desulfocapsa through the disproportionation of bisulfite. These novel insights could not have been obtained through the traditional assay methods currently used in the management of MIC and indicate the promise of molecular methods in future MIC investigations. Molecular Microbiological Methods Applied on Ship Ballast Tank Samples.
Anne Heyer, Fraddry D'Souza, Arjan Mol and Hans de Wit. Microbiologically influenced corrosion of steel is a serious problem in the marine environment including offshore and shipping industries. The uses of molecular techniques and electrochemical techniques for evaluating microbial corrosion effects have continued to gain importance. This chapter summarizes a large- scale approach of a simultaneous investigation of corrosion and microbial community within a practical ship ballast tank environment. The corrosion behaviour of ASTM A1. EH3. 6 exposed to natural seawater was investigated at two different height levels of the ship ballast tank- sediment and immersion zone. Biofilm samples were scraped from exposed corrosion coupons and subjected to DGGE of PCR- amplified 1.
S r. RNA genes and sequence analysis to characterize the microbial community developed on the sidewall of ship ballast tanks as well as the water phase. In parallel, corrosion was monitored by open circuit potential and linear polarization resistance measurements, indicating a difference in corrosion behaviour at sediment and immersion zone and related to the variation in bacterial community changes. This chapter demonstrates the problem of attached biofilms in the corrosion of ship ballast tanks, which is characterized by a combination of electrochemical and molecular tools. Molecular Characterization of Microbial Communities Associated with Accelerated Low Water Corrosion (ALWC) on European Harbor Structures. Florence Marty, Mark van Loosdrecht and Gerard Muyzer. The microbial communities associated with different corrosion deposit layers retrieved from a harbour steel structure affected by ALWC (Accelerated Low Water Corrosion) were determined by 1.
S r. DNA PCR- DGGE analysis. Comparative analysis of populations associated with ALWC layers and NLWC (Normal Low Water Corrosion) layers evidenced clear differences in the structure and composition of the communities.
Dominant phylotypes related to sulphate- reducing bacteria pertaining to Desulfobacteraceae, Desulfobulbaceae and Desulfovibrionaceae were identified in both type of deposits (fraction of 5. NLWC and ALWC communities, respectively). Phylotypes related to sulphur- oxidizing bacteria belonging to Alpha- , Gamma- and Epsilonproteobacteria were unique to ALWC deposits, while phylotypes related to oxygenic photosynthetic microorganisms (cyanobacteria, diatoms) were only retrieved in NLWC deposits. This suggested that biologically- mediated sulphur cycle was the dominant process within ALWC deposits vs. NLWC deposits. Since most of the members identified are heterotrophs, it is speculated that organic carbon may be available through water pollution and/or proliferation of photosynthetic biomass. Differences in oxygen concentration between ALWC and NLWC areas due to photosynthetic activities in NLWC may be an important factor contributing to the acceleration of corrosion by the mechanism of differential aeration.
Application of the q. PCR Technique for SRB Quantification in Samples from the Oil and Gas Industries.
Mariana Galvão and Márcia Lutterbach. Microorganisms can grow in many different types of fuels and industrial facilities.
Microorganisms may grow in the presence of oxygen (aerobic conditions) or in its absence (anaerobic conditions), feeding primarily on hydrocarbon fuel, minerals and other impurities in the water. In practice, the bottoms of fuel storage tanks or even tanks of buses and trucks contain enough water to allow for significant microbial growth. Microbial activity leads to the production of biomass (fouling), which can be deposited at the bottom of the tank.
ORAL PROBIOTICS: Fighting Tooth Decay, Periodontal Disease and Airway Infections Using Friendly Bacteria. DO YOU HAVE PERIODONTAL DISEASE – ALSO CALLED GINGIVITIS? HOW ABOUT DENTAL DECAY? DID YOU KNOW THAT GUM DISEASE IS NOW LINKED TO ARTHRITIS?
AND HEART DISEASE? AND CHRONIC FATIGUE AND OTHER AILMENTS? THIS BOOK WILL TELL YOU WHY.
IT WILL ALSO SHOW YOU HOW TO REVERSE THE PROBLEM SIMPLY AND INEXPENSIVELY. THIS BOOK WILL DESCRIBE THE SCIENCE OF ORAL BACTERIA. IT WILL ALSO EXPLAIN HOW TO SUPPLEMENT WITH ORAL PROBIOTICS. AND THE LATEST SCIENTIFIC EVIDENCE ON THE BEST WAYS TO KEEP OUR MOUTHS FROM INFECTING OUR BODIES. ALL FOR LESS THAN A CUP OF COFFEE WILL COST.
ORAL PROBIOTICS: Fighting Tooth Decay, Periodontal Disease and Airway Infections Using Friendly BacteriaÂ Every mouth is full of bacteria, yeasts, fungi and viruses. Can we stop these infectious microorganisms from making us sick? Today, with millions dawning face masks, washing with antibacterial soaps, and sanitizing classrooms, hospitals and other public places, we need new solutions. Peer- reviewed research from some of the world’s foremost microbiologists is now demonstrating that the probiotic bacteria in our mouth can be used to reduce or prevent infections from invading the body’s internal tissues.
Our oral probiotics can be our first line of defense against some of the most dangerous diseases, including those caused by aggressive bacteria, flu viruses and yeasts. In this groundbreaking book, the author details the newest research revealing the cooperative roles friendly oral bacteria play within our immune system to fight infection and prevent disease. Guidance on supplementation and on how to encourage the growth of our resident oral probiotic colonies is also described. A must read for anyone wanting to strengthen the immune system and stay healthy.
To order the updated print book: Get Print Book. Or instantly download the updated Ebook: Download Ebook. Download Ebook. Download the Ebook. Table of Contents. Introduction. One: Microbes in Every Mouth. The Germ Theory. The Dawning of the Probiotic Era.
The Anti- Microbial Mouth. How Do Bacteria Become Resistant? Foodborne Illness. Microorganism Mania. How Many Bacteria Does it Take to Get Sick?
Two: The Oral Immune System. The Non- specific Immune System. Humoral Immune Response.
Cell- Mediated Immune Response. The Probiotic Immune Response.
Oral and Nasal Immunity. Stimulating the Oral Immune System. Digestive Immunity. Immune Colonization. Three: The Probiotic Gatekeepers. Probiotic Inoculation.
Housing the Resident Strains. Territorial Behavior. Four: Oral Probiotics and Disease. Summary of Probiotic Mechanisms. Oral Cavity Probiotic Research. Periodontal Disease and Dental Caries. Bacterial Infections.
Allergies. Intestinal Permeability. Ulcers. Vaginosis and Vaginitis.
Candida Infections. Baby Colic. Ear Infections. Anorexia Nervosa. Keratoconjunctivitis. Viral Infections. Halitosis (Bad Breath)Respiratory Infections. Rebuilding the Immune System.
Five: Meet Your Oral Bacteria. Species and Strains. The Bad and Not So Bad Guys.
Actinobacillus actinomycetemcomitans. Actinomyces sp. Clostridium sp.
Corynebacterium sp. Entamoeba gingivalis. Enterococcus faecalis (formerly Streptococcus faecalis)Escherichia coli and other Enterobacteriaceae. Haemophilus sp. Helicobacter pylori.
Mycoplasmas. Neisseria meningitides. Pseudomonas aeruginosa. Staphylococcus aureus. Staphylococcus epidermidis. Streptococcus mitis. Streptococcus pyrogenes. Streptococcus pneumoniae.
Streptococcus mutans. Treponema Denticola. The Good Guys. Probiotic Streptococci. Streptococcus salivarius. Streptococcus thermophilus. The Lactobacilli.
Lactobacillus salivarius. Lactobacillus reuteri. Lactobacillus acidophilus. Lactobacillus helveticus.
Lactobacillus casei. Lactobacillus rhamnosus. Lactobacillus plantarum. Lactobacillus bulgaricus. Lactobacillus brevis. Oral Probiotic Supplements.
Six: Oral Probiotic Supplement Options. Dosage Considerations. Oral Flora Residents. Probiotic Nourishment: Prebiotics.
The Probiotic- Friendly Diet. Probiotic Hydration. Probiotic Foods. Probiotic Oral Hygiene. Problems with Gum Disease. How to Reduce Our Risk of Periodontal Disease.
The Healthy Oral Regimen. Probiotic Night Owls. In Conclusion. References and Bibliography.